EDVOTEK Using CRISPR to Treat Cystic Fibrosis

EDVOTEK Using CRISPR to Treat Cystic Fibrosis

EP00052457

Product code: EP00052457

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

  • Group Size: For 8 Lab Groups
  • Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
  • Time Required: Complete in 45 minutes

The kit includes:

  • Instructions,
  • Ready-to-Load QuickStrip™ DNA Samples
  • UltraSpec-Agarose™
  • Electrophoresis Buffer (50X)
  • 10X Gel Loading Solution,
  • FlashBlue™ DNA Stain

Additional required equipment & materials (not supplied with the kit):

  • DNA Electrophoresis tank and accessories including casting trays and special power supply
  • Micropipettes: 5-50 µl (Optional)
  • Balance
  • White Light Box
  • Microwave or Hot Plate

*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*

  • An excellent activity to demonstrate genetics and Cystic Fibrosis Diagnostics and Treatment
  • A-Level Genetics: Principles & Applications of Electrophoresis
  • When visualising completed gels using a white light box, cover the light box with clingfilm to prevent any damage.

Further Information

Age Recommended from
14 Years
Age Suitable from
14 Years
Brand
Edvotek
Experiment Title
Using CRISPR to Treat Cystic Fibrosis
Number of Lab Groups per Kit
8 Lab Groups
Product Type
Electrophoresis Kit
Storage
Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
Time Required
Complete in 45 minutes

Documents

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

  • Group Size: For 8 Lab Groups
  • Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
  • Time Required: Complete in 45 minutes

The kit includes:

  • Instructions,
  • Ready-to-Load QuickStrip™ DNA Samples
  • UltraSpec-Agarose™
  • Electrophoresis Buffer (50X)
  • 10X Gel Loading Solution,
  • FlashBlue™ DNA Stain

Additional required equipment & materials (not supplied with the kit):

  • DNA Electrophoresis tank and accessories including casting trays and special power supply
  • Micropipettes: 5-50 µl (Optional)
  • Balance
  • White Light Box
  • Microwave or Hot Plate

*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*

  • An excellent activity to demonstrate genetics and Cystic Fibrosis Diagnostics and Treatment
  • A-Level Genetics: Principles & Applications of Electrophoresis
  • When visualising completed gels using a white light box, cover the light box with clingfilm to prevent any damage.

Further Information

Age Recommended from
14 Years
Age Suitable from
14 Years
Brand
Edvotek
Experiment Title
Using CRISPR to Treat Cystic Fibrosis
Number of Lab Groups per Kit
8 Lab Groups
Product Type
Electrophoresis Kit
Storage
Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
Time Required
Complete in 45 minutes

Documents

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